Anthocyanin Utilization Of Mangsian Fruit Extract (Phyllanthus Reticulatus) As An Alternative Colorant In Blood Meal Preparation (SADT)

Abstract

The peripheral blood smear (SADT) examination is important for medical diagnosis. It involves staining blood preparations to allow clear visualization of peripheral blood cells, which include erythrocytes, leukocytes and platelets. One of the guarantees of the validity of SADT results is the quality of the SADT stain. Giemsa stain has disadvantages related to toxicity, availability of hazardous chemicals, and negative impact on the environment. Anthocyanins, natural pigment compounds found in many fruits, flowers, and vegetables, show great potential as natural colorants in SADT staining. One example is anthocyanins from mangsian fruit (Phyllanthus reticulatus), as a safer and environmentally friendly alternative. This plant contains tannic acid, terpenoids, flavonoids, phenolic compounds, and steroids. This study aims to assess the effectiveness of anthocyanin extract of mangsian fruit (Phyllanthus reticulatus) as an alternative coloring in peripheral blood smear preparations (SADT). The method used is experimental research, which is research that aims to determine the effects that arise as a result of certain treatments on a variable, by testing concentrations of 50%, 40%, 30%, and 20%. The results showed that anthocyanin extract was less effective in staining blood cells, macroscopically and microscopically, erythrocyte and leukocyte cells could not absorb the color of the dye properly.