COMPARISON OF DRYING TIME OF REAGENTS BETWEEN EOSIN 2% REAGENTS AND NATURAL REAGENTS FROM IXORA COCCINEA FLOWERS AND BOUGAINVILLE FLOWERS FOR SOIL TRANSMITTED HELMINTH WORM EGGS EXAMINATION

Abstract

Eosin 2% reagent is commonly used to make stool preparations, but this reagent dries quickly so that the stool preparation cannot be left for a long time. The red Ixora coccinea flowers and the red Bougainvillea flowers are red in color which is similar to eosin reagent. The red Ixora coccinea is renowned for its anthocyanin content, especially pelargonidin glycosides, which contribute to its bright color. Betalain compounds betasianin and betaxanthin are the color pigments in bougainvillea flowers. Betalains are water-soluble pigments that can be divided into betacyanins (red color) and betaxanthins (yellow color). This study aims to comparison of drying time of reagents between eosin 2% reagents and natural reagents from ixora coccinea flowers and bougainville flowers for soil transmitted helminth worm eggs examination. Complete Randomised Design (CRD) is the research methodology used in this quasi experimental study, to see the comparison of eosin, Ixora coccinea flower’s juice and red bougainvillea flower’s repeat three more times and Anova used for data analysis. The average drying result of eosin reagent was 14.7 minutes, the average drying result of ixora coccinea flower juice was 24 minutes and the average drying result of bougainvillea flower juice was 29.3 minutes. From the research results it can be concluded that annova test there was a significant difference in the drying time of the reagent on the soil transmitted helminth egg preparation with P value 0, 000 ˂ alpha value of 0.05.